Rahmann H, Körtje K H, Seybold V, Rösner H
Zoological Institute, University of Stuttgart-Hohenheim, Hohenheim, F.R.G.
Indian J Biochem Biophys. 1990 Dec;27(6):420-4.
By means of newly developed electron microscopical techniques (electron spectroscopic imaging, ESI; electron energy loss spectroscopy, EELS; immunogold labelling) a specific accumulation of endogenous calcium within the synaptic cleft and a distinct localization of a high-affinity Ca(2+)-ATPase at the inner sides of the pre- and postsynaptic membrane of nerve cells from fish brain have been demonstrated. Additionally, a differentiation-dependent expression of polysialoganglioside epitopes on the outer surface of nerve terminals in clustered arrangements was demonstrated using their ultracytochemical detection by means of the monoclonal antibody Q211. These results which are in agreement with parallel biochemical investigations on modulatory effects of exogenous gangliosides on a high-affinity Ca(2+)-ATPase in the CNS of vertebrates support our hypothesis that Ca(2+)-ganglioside complexes act as modulators for the processes of synaptic transmission and long-term neuronal adaptations.
通过新开发的电子显微镜技术（电子光谱成像，ESI；电子能量损失谱，EELS；免疫金标记），已证明鱼脑神经元突触间隙内有内源性钙的特异性积累，以及高亲和力Ca(2+)-ATP酶在突触前膜和突触后膜内侧的独特定位。此外，利用单克隆抗体Q211的超细胞化学检测，证明了神经末梢外表面呈簇状排列的多唾液酸神经节苷脂表位的分化依赖性表达。这些结果与关于外源性神经节苷脂对脊椎动物中枢神经系统高亲和力Ca(2+)-ATP酶调节作用的平行生化研究结果一致，支持了我们的假设，即Ca(2+)-神经节苷脂复合物作为突触传递和长期神经元适应性过程的调节剂。
