Aboagye-Mathiesen G, Tóth F D, Juhl C, Nørskov-Lauritsen N, Petersen P M, Zachar V, Ebbesen P
Danish Cancer Society, Department of Virus and Cancer, Aarhus C.
J Gen Virol. 1991 Aug;72 ( Pt 8):1871-6. doi: 10.1099/0022-1317-72-8-1871.
Human placental trophoblast cultures produce a mixture of interferons (IFNs) when challenged with Sendai virus. High-performance dye-ligand and immunoaffinity chromatography of a trophoblast IFN (tro-IFN) preparation enabled the isolation of three antigenically distinct IFNs, alpha I, alpha II 1 and beta, with Mrs of 16K, 22K and 24K respectively, by reducing and non-reducing SDS-PAGE. The major IFN, responsible for 75% of the total antiviral activity, was tro-IFN-beta, with the remaining activity being due to tro-IFN-alpha I and tro-IFN-alpha II 1, as determined by an antiviral neutralization test using specific anti-human IFN antibodies. The antiviral activities of the tro-IFNs were stable at pH 2.0 for 24 h and tro-IFN-alpha II 1 and -beta were shown to be glycoproteins. The three tro-IFNs showed different antiviral activities when assayed on human and bovine cell species; tro-IFN-alpha I and alpha II 1 protected both human and bovine (MDBK) cells from virus infection, whereas tro-IFN-beta showed a high degree of species specificity, protecting only the human cell types tested.
人胎盘滋养层细胞培养物在受到仙台病毒攻击时会产生多种干扰素（IFN）。通过对滋养层干扰素（tro-IFN）制剂进行高效染料配体和免疫亲和层析，利用还原和非还原SDS-PAGE分离出三种抗原性不同的干扰素，分别为α I、α II 1和β，其相对分子质量分别为16K、22K和24K。通过使用特异性抗人干扰素抗体的抗病毒中和试验确定，主要的干扰素是tro-IFN-β，占总抗病毒活性的75%，其余活性归因于tro-IFN-α I和tro-IFN-α II 1。tro-IFN的抗病毒活性在pH 2.0条件下24小时保持稳定，并且tro-IFN-α II 1和-β被证明是糖蛋白。当在人和牛细胞种类上进行检测时，这三种tro-IFN表现出不同的抗病毒活性；tro-IFN-α I和α II 1可保护人和牛（MDBK）细胞免受病毒感染，而tro-IFN-β表现出高度的种属特异性，仅保护所检测的人细胞类型。
