Yamaguchi Hiroshi, Oda Shuji, Kondo Yoshinori, Ono Akira, Tanaka Yoshiyuki
Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):113-4. doi: 10.1093/nass/48.1.113.
The DNA duplex with tandem T x T mismatches has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T x T mismatches, TT10:d(CGCGTTGTCC) x d(GGACTTCGCG). From two-dimensional (2D) 1H-1H NOESY spectrum, we were able to trace sequential NOE walks between base protons and anomeric protons (H1'), and assigned all the base protons and anomeric protons. These results indicate that the overall structure of the duplex is a right-handed duplex, with two successive T x T mismatches stacked in the helix.
已通过核磁共振光谱法研究了具有串联T×T错配的DNA双链体。在本研究中，我们合成了具有两个连续T×T错配的十聚体双链体，即TT10：d（CGCGTTGTCC）×d（GGACTTCGCG）。从二维（2D）1H-1H NOESY光谱中，我们能够追踪碱基质子与异头质子（H1'）之间的连续NOE路径，并对所有碱基质子和异头质子进行了归属。这些结果表明，双链体的整体结构是右手双链体，两个连续的T×T错配堆积在螺旋中。
