Yamaguchi Hiroshi, Oda Shuji, Kojima Chojiro, Ono Akira, Kondo Yoshinori, Tanaka Yoshiyuki
Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):199-200. doi: 10.1093/nass/49.1.199.
The DNA duplex with tandem T x T mismatches in the presence of Hg(II) has been studied by NMR spectroscopy. For this study, we synthesized decamer duplex with two successive T x T mismatches (TT10). From two-dimensional (2D) 1H-1H NOESY spectrum of TT10 in complex with Hg(II), we were able to trace sequential NOE walks between base protons and anomeric protons (H1'), and assigned all of them. Based on these assignments and NOESY spectra, we could assigned all the non-exchangeable protons of TT10 in the presence of Hg(II).
通过核磁共振光谱法研究了在汞（II）存在下具有串联T×T错配的DNA双链体。为了这项研究，我们合成了具有两个连续T×T错配的十聚体双链体（TT10）。从TT10与汞（II）复合物的二维（2D）1H-1H NOESY光谱中，我们能够追踪碱基质子和异头质子（H1'）之间的连续核Overhauser效应（NOE）路径，并对所有这些质子进行了归属。基于这些归属和NOESY光谱，我们能够归属汞（II）存在下TT10的所有非交换质子。
