一种从结构化文库中获得的连接酶核酶。

A ligase ribozyme obtained from a structured pool.

作者信息

Yoshioka Wataru, Ikawa Yoshiya, Jaeger Luc, Inoue Tan

机构信息

Department of Chemistry, Graduate School of Science, Kyoto University, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):209-10. doi: 10.1093/nass/48.1.209.

DOI:10.1093/nass/48.1.209

PMID:17150552

Abstract

Here we report the in vitro selection experiment employed to construct a catalytic site on a self-folding RNA. To practice the selection, the sequence of 30 nucleotides was located in close proximity of the putative reaction site in a derivative of a naturally occurring RNA with a well known 3D structure. The acquired catalytic RNA retained the starting 3D structure and was successfully reconstructed according to its modular structure.

摘要

在此，我们报告了用于在自我折叠RNA上构建催化位点的体外筛选实验。为了进行筛选，在具有已知三维结构的天然存在的RNA衍生物中，30个核苷酸的序列位于假定反应位点的附近。获得的催化RNA保留了起始三维结构，并根据其模块化结构成功重建。