Ikebukuro Kazunori, Yoshida Wataru, Sode Koji
Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):231-2. doi: 10.1093/nass/48.1.231.
A novel DNA detection system has been developed using the DNA aptamer inhibiting thrombin activity. The complementary strand to the target DNA was inserted into the loop region of the G-quartet structure of this adapter aiming at the disruption of it by the hybridization of the target DNA with its complementary strand in the aptamer. Using this modified aptamer, we were able to detect the target DNA by measuring thrombin activity.
一种利用抑制凝血酶活性的DNA适配体开发的新型DNA检测系统已被研制出来。目标DNA的互补链被插入到该适配体的G-四联体结构的环区域中，目的是通过目标DNA与其在适配体中的互补链杂交来破坏该结构。使用这种修饰后的适配体，我们能够通过测量凝血酶活性来检测目标DNA。
