Ikebukuro Kazunori, Yoshida Wataru, Sode Koji
Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):83-4. doi: 10.1093/nass/49.1.83.
A novel sensing probe for adenosine has been developed using the thrombin-inhibiting DNA aptamer fused with the adenosine-binding DNA aptamer. The adenosine aptamer was inserted into the G-quartet structure of the thrombin aptamer to change its inhibitory activity to thrombin upon binding of the adenosine aptamer part to adenosine. This designed aptamer had lower inhibitory activity than that of the original thrombin aptamer, and it was increased in the presence of adenosine. CD spectra of this designed aptamer indicated that its structure was changed by its binding to adenosine. Using this designed aptamer, we were able to detect adenosine by measuring thrombin activity in homogeneous assay.
一种用于检测腺苷的新型传感探针已被开发出来，它是将与腺苷结合的DNA适配体融合到凝血酶抑制性DNA适配体上。将腺苷适配体插入凝血酶适配体的G-四联体结构中，使其在腺苷适配体部分与腺苷结合时改变对凝血酶的抑制活性。这种设计的适配体比原始凝血酶适配体具有更低的抑制活性，并且在腺苷存在时其抑制活性会增加。这种设计的适配体的圆二色光谱表明，其结构因与腺苷结合而发生了变化。利用这种设计的适配体，我们能够通过在均相分析中测量凝血酶活性来检测腺苷。
