Ihara Toshihiro, Mukae Motoko, Tabara Miyuki, Kitamura Yusuke, Jyo Akinori
Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
Nucleic Acids Symp Ser (Oxf). 2005(49):41-2. doi: 10.1093/nass/49.1.41.
Anthracene readily forms photoadducts (anthracene dimers). The reaction generally requires close proximity and certain spatial alignment of both reaction partners. DNA could provide an ideal scaffold for accelerating the reaction, photocyclic addition. We synthesized a number of anthracene-DNA conjugates. The sequences of the conjugates, 5'AntODN and 3'AntODN, were designed to bind adjacent sequences of the template with the anthracene units directed such that they stacked with each other. The conjugates were only dimerized in the presence of the template by light irradiation. The efficiency of dimerization was dependent on the structure of the conjugates and affected by one-base displacement in the template sequence.
蒽很容易形成光加合物（蒽二聚体）。该反应通常需要两个反应伙伴紧密靠近并具有一定的空间排列。DNA可以为加速光环化加成反应提供理想的支架。我们合成了许多蒽-DNA缀合物。缀合物5'AntODN和3'AntODN的序列被设计成与模板的相邻序列结合，蒽单元的方向使得它们相互堆叠。通过光照射，缀合物仅在模板存在的情况下发生二聚化。二聚化效率取决于缀合物的结构，并受模板序列中一个碱基位移的影响。
