Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
Org Biomol Chem. 2010 Nov 7;8(21):4843-8. doi: 10.1039/c0ob00282h. Epub 2010 Aug 24.
We prepared an oligodeoxyribonucleotide conjugate (5-3ant(2)18) carrying two anthracenes, each of which was tethered to both ends of the conjugate through hexamethylene linker chains. The conjugate has a mirror repeat of two heptamer sequences, such that it forms a bimolecular triplex with the single stranded target, forming a two-fold U-shaped conformation. The conformation of the conjugate in its triplex structure could be frozen instantaneously by circularization through photodimerization of the anthracenes. Compared with the duplex formation of linear probes with relevant sequences, bimolecular triplex formation of 5-3ant(2)18 shows a unique feature in its target recognition; it binds the target tightly, yet still retains high sequence selectivity. Circularization of 5-3ant(2)18 by UV photoirradiation was verified as the probe reaction for a DNA assay. The probe reaction could be performed in a few seconds over a wide range of temperatures, at least between 0 and 25 °C. In addition, the reaction could be regarded as a reversible method for the preparation of circular DNA that shows higher affinity for the target.
我们制备了一种带有两个蒽基团的寡脱氧核苷酸缀合物（5-3ant(2)18），每个蒽基团通过六亚甲基连接链连接到缀合物的两端。该缀合物具有两个七聚体序列的镜像重复，因此它与单链靶标形成双分子三联体，形成两倍 U 形构象。通过蒽的光二聚化使缀合物在其三聚体结构中环化，可以瞬间冻结其构象。与具有相关序列的线性探针的双链体形成相比，5-3ant(2)18 的双分子三聚体形成在其靶标识别中表现出独特的特征；它紧密结合靶标，但仍保持高序列选择性。UV 光辐照下的 5-3ant(2)18 环化被验证为 DNA 分析的探针反应。该探针反应可以在很宽的温度范围内在几秒钟内进行，至少在 0 到 25°C 之间。此外，该反应可以被视为一种用于制备对靶标具有更高亲和力的环状 DNA 的可逆方法。
