功能核酸与金属相互作用的核磁共振光谱分析及其溶液结构分析。

NMR spectroscopic analyses of functional nucleic acids-metal interaction and their solution structure analyses.

作者信息

Tanaka Yoshiyuki, Yamaguchi Hiroshi, Oda Shuji, Kojima Chojiro, Ono Akira, Taira Kazunari, Kondo Yoshinori

机构信息

Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai 980-8578, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):51-2. doi: 10.1093/nass/49.1.51.

DOI:10.1093/nass/49.1.51

PMID:17150628

Abstract

We have studied complexations of functional nucleic acids and metal ions, by means of NMR spectroscopy. In the case of hammerhead ribozymes, they have a metal ion-binding motif in their core sequences. Upon the metallation of N7 of a guanosine in an RNA duplex modelled after hammerhead ribozymes, 20 ppm higher field shift of N7 was observed in 1-dimensional (1D) 15N NMR spectra. It was found that metal ion binding to nucleobases were detectable with 15N NMR spectroscopy.

摘要

我们通过核磁共振光谱法研究了功能性核酸与金属离子的络合作用。就锤头状核酶而言，它们在核心序列中具有金属离子结合基序。在以锤头状核酶为模型的RNA双链体中，鸟苷的N7发生金属化后，在一维（1D）15N核磁共振光谱中观察到N7有20 ppm的高场位移。研究发现，利用15N核磁共振光谱可以检测到金属离子与核碱基的结合。