Synthesis of cross-linked circular DNAs using Hüisgen reaction.
作者信息
Nakane Masanori, Ichikawa Satoshi, Matsuda Akira
机构信息
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, kita-ku, Sapporo 060-0812, Japan.
出版信息
Nucleic Acids Symp Ser (Oxf). 2005(49):189-90. doi: 10.1093/nass/49.1.189.
PMID:17150697
Abstract
Triazole-cross-linked ODNs were synthesized using Hüisgen reaction with 21 mer hairpin DNA possessing N-3 azidoethylthymidine and N-3 propargylthymidine at the 3' and 5'-terminals. Newly synthesized ODNs revealed thermally stable and their structures nearly retained those of non-cross-liked ODNs. This strategy is quite effective to prepare cross-linked circular ODNs.
摘要
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