使用反基因RNA抑制染色体DNA的转录。

Inhibiting transcription of chromosomal DNA using antigene RNAs.

作者信息

Janowski Bethany A, Corey David R

机构信息

Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9041, USA.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):367-8. doi: 10.1093/nass/49.1.367.

DOI:10.1093/nass/49.1.367

PMID:17150786

Abstract

Transcription start sites are critical switches for converting recognition of chromosomal DNA into synthesis of RNA. We report that antigene peptide nucleic acid (agPNA) oligomers or antigene RNA (agRNA) duplexes can target transcription start sites and block gene expression. Our data suggest that chromosomal DNA within cells is readily accessible to recognition by synthetic agents. agPNAs and agRNAs will be useful tools for silencing gene expression and will provide insights into natural mechanisms for controlling transcription.

摘要

转录起始位点是将染色体DNA识别转化为RNA合成的关键开关。我们报告称，反基因肽核酸（agPNA）寡聚物或反基因RNA（agRNA）双链体可靶向转录起始位点并阻断基因表达。我们的数据表明，细胞内的染色体DNA很容易被合成试剂识别。agPNA和agRNA将成为沉默基因表达的有用工具，并将为控制转录的天然机制提供见解。