一种具有2'-O-(2-氰基乙氧基甲基)保护基团的新型RNA合成方法。

A novel RNA synthetic method with a 2'-O-(2-cyanoethoxymethyl) protecting group.

作者信息

Shiba Yoshinobu, Kitagawa Hidetoshi, Masutomi Yutaka, Ishiyama Kouichi, Ohgi Tadaaki, Yano Junichi

机构信息

Discovery Research Laboratories, Nippon Shinyaku Co. Ltd., 3-14-1 Sakura, Tsukuba City, Ibaraki 305-0003, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2006(50):11-2. doi: 10.1093/nass/nrl006.

DOI:10.1093/nass/nrl006

PMID:17150792

Abstract

A novel method for the synthesis of RNA oligomers with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group has been developed. The new method allows the synthesis of oligonucleotides with an efficiency and final purity comparable to that obtained in DNA synthesis.(1) In addition, the CEM method has the potential for application to the synthesis of very long RNA oligonucleotides.

摘要

已经开发出一种以2-氰基乙氧基甲基（CEM）作为2'-羟基保护基团来合成RNA寡聚物的新方法。该新方法能够合成效率和最终纯度与DNA合成相当的寡核苷酸。（1）此外，CEM方法有应用于合成非常长的RNA寡核苷酸的潜力。