Pap A, Boros L, Hajnal F
First Department of Medicine, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Pancreas. 1991 Jul;6(4):412-8. doi: 10.1097/00006676-199107000-00007.
The essential role of cholecystokinin (CCK) in pancreatic regeneration after pancreatitis or resection has been supposed, but not yet clearly demonstrated. In rats, 6-8 weeks after 60% distal resection of the pancreas a gradual increase in pancreatic weight and contents of DNA, protein, trypsin, chymotrypsin and amylase, occurred (there was no increase in lipase); Pancreatic regeneration stopped thereafter. Nonparallel increases in enzyme values were similar to those seen after CCK administration. Indeed, basal CCK levels increased significantly by the 6th week and declined thereafter. A one month s.c. administration of CCK-octapeptide (CCK-8) (3 x 300 ng/kg/d) accelerated regeneration in the first month, but it had almost no effect during the second or third postoperative months. A two week s.c. administration of a specific CCK antagonist, CR 1409 (3 x 4 mg/kg/d) totally prevented regeneration by the fifth and sixth weeks, but did not diminish pancreatic weight or DNA and protein contents during the first two weeks. Alcohol administration (12 g/kg/d) reduced CCK release and prevented pancreatic regeneration during the three-month experimental period. These data indicate that CCK has an essential role in pancreatic regeneration and that the deleterious effect of alcohol on regeneration involves inhibition of CCK release.
胆囊收缩素（CCK）在胰腺炎或胰腺切除术后胰腺再生中的重要作用已被推测，但尚未得到明确证实。在大鼠中，胰腺远端60%切除术后6 - 8周，胰腺重量以及DNA、蛋白质、胰蛋白酶、糜蛋白酶和淀粉酶含量逐渐增加（脂肪酶含量未增加）；此后胰腺再生停止。酶值的非平行增加与给予CCK后所见相似。实际上，基础CCK水平在第6周时显著升高，此后下降。皮下注射CCK - 八肽（CCK - 8）（3×300 ng/kg/d）持续1个月，在第一个月加速了再生，但在术后第二个月或第三个月几乎没有效果。皮下注射一种特异性CCK拮抗剂CR 1409（3×4 mg/kg/d）持续2周，到第5周和第6周时完全阻止了再生，但在前两周并未减轻胰腺重量或DNA和蛋白质含量。给予酒精（12 g/kg/d）在三个月的实验期内减少了CCK释放并阻止了胰腺再生。这些数据表明CCK在胰腺再生中起重要作用，并且酒精对再生的有害作用涉及抑制CCK释放。
