人工限制性DNA切割器对巨大DNA的位点选择性水解

Site-selective hydrolysis of huge DNA by artificial restriction DNA cutter.

作者信息

Yamamoto Yoji, Miura Kazuyuki, Komiyama Makoto

机构信息

Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2006(50):265-6. doi: 10.1093/nass/nrl132.

DOI:10.1093/nass/nrl132

PMID:17150919

Abstract

Genomic DNAs of lambda phage (48.5 kbp) and E. coli (4.6 Mbp) were successfully cleaved at the target site by artificial restriction DNA cutter (ARCUT), which we recently developed by combining Ce(IV)/EDTA and PNA (peptide nucleic acid). PNA satisfactorily invaded the target site in such huge DNAs and activate there for Ce(IV)/EDTA-induced scission.

摘要

通过人工限制性DNA切割酶（ARCUT）成功在目标位点切割了λ噬菌体（48.5 kbp）和大肠杆菌（4.6 Mbp）的基因组DNA，ARCUT是我们最近通过将Ce(IV)/EDTA与肽核酸（PNA）结合而开发的。PNA能够令人满意地侵入如此巨大的DNA中的目标位点，并在那里激活以实现Ce(IV)/EDTA诱导的切割。