由铈（IV）/乙二胺四乙酸（EDTA）和含磷酸基团的肽核酸组成的高活性人工限制酶——磷酸促进作用与入侵复合物结构之间的关系

Highly active artificial restriction enzyme composed of Ce(IV)/EDTA and PNA bearing phosphate group--relationship between the promotion by phosphate and the structure of invasion complex.

作者信息

Aiba Yuichiro, Yamamoto Yoji, Komiyama Makoto

机构信息

Research center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2006(50):255-6. doi: 10.1093/nass/nrl127.

DOI:10.1093/nass/nrl127

PMID:17150914

Abstract

Recently, we developed artificial restriction DNA cutter (ARCUT) composed of pseudo-complementary peptide nucleic acid (pcPNA) and Ce(IV)/EDTA complex (EDTA = ethylenediamine-N,N,N',N'-tetraacetate). Here we promoted the site-selective hydrolysis by attaching phosphate groups to the pcPNAs. The promotion by the phosphates increased with decreasing length of the gap-like site. Furthermore, the scission was successful even when phosphate groups were introduced to 0 base-gap system.

摘要

最近，我们开发了由假互补肽核酸（pcPNA）和铈（IV）/乙二胺四乙酸络合物（EDTA = 乙二胺-N，N，N'，N'-四乙酸）组成的人工限制性DNA切割器（ARCUT）。在此，我们通过将磷酸基团连接到pcPNA上来促进位点选择性水解。磷酸基团的促进作用随着间隙样位点长度的减小而增强。此外，即使将磷酸基团引入0碱基间隙系统，切割也取得了成功。