Site-specific gene manipulation of fluorescent proteins using artificial restriction DNA cutter.
作者信息
Katada Hitoshi, Ren Yi, Shigi Narumi, Komiyama Makoto
机构信息
Research Center for Advanced Science and Technology, The University of Tokyo, Meguro, Tokyo 153-8904, Japan.
出版信息
Nucleic Acids Symp Ser (Oxf). 2008(52):483-4. doi: 10.1093/nass/nrn245.
PMID:18776464
Abstract
Two of three amino acid residues, which compose the chromophore of the enhanced green fluorescent protein (EGFP), were converted to others by using artificial restriction DNA cutter (ARCUT). The vector prepared by ARCUT was easily connected with the insert by using oligonucleotide additive and resultant fluorescent protein such as blue fluorescent protein (BFP) was successfully expressed in cells.
摘要
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