将小鼠卵丘细胞核暴露于猪卵质提取物中可消除TATA盒蛋白与染色质的结合，但对DNA甲基化没有影响。
Exposure of mouse cumulus cell nuclei to porcine ooplasmic extract eliminates TATA box protein binding to chromatin, but has no effect on DNA methylation.
作者信息
Tong Guo Qing, Heng Boon Chin, Ng Soon Chye
机构信息
Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore.
出版信息
J Assist Reprod Genet. 2006 Nov-Dec;23(11-12):413-9. doi: 10.1007/s10815-006-9083-8. Epub 2006 Dec 7.
PURPOSE
The low cloning efficiency with SCNT is due to incomplete or partial reprogramming of the donor somatic cell nuclei after microinjection into the enucleated oocyte. A possible solution may be to initiate nuclear reprogramming prior to SCNT.
METHODS
Pre-exposure of donor somatic cell nuclei to a novel porcine ooplasmic extract prior to microinjection could possibly extend the duration of exposure to ooplamic nuclear reprogramming factors. The effects of the porcine ooplamic extract on two major markers of nuclear preprogramming: (1) TATA box protein binding to chromation and (2) DNA methylation was investigated.
RESULTS
The results showed that pre-exposure of mouse cumulus cell nuclei to porcine ooplamic extract drastically reduced TATA box protein binding to chromatin, but had no effect on DNA methylation.
CONCLUSIONS
Pre-exposure to the porcine ooplasmic extract had some limited effects on nuclear reprogramming. Whether this can lead to enhanced cloning efficiency needs to be further investigated.
目的
体细胞核移植（SCNT）的克隆效率较低，这是由于将供体体细胞的细胞核显微注射到去核卵母细胞后，供体细胞核的重编程不完全或部分重编程所致。一种可能的解决办法或许是在进行体细胞核移植之前启动细胞核重编程。
方法
在显微注射之前，使供体体细胞的细胞核预先暴露于一种新型猪卵质提取物中，这有可能延长细胞核暴露于卵质重编程因子的时间。研究了猪卵质提取物对细胞核预重编程的两个主要标志物的影响：（1）TATA盒蛋白与染色质的结合，以及（2）DNA甲基化。
结果
结果表明，将小鼠卵丘细胞核预先暴露于猪卵质提取物中，会显著降低TATA盒蛋白与染色质的结合，但对DNA甲基化没有影响。
结论
预先暴露于猪卵质提取物对细胞核重编程有一定的有限影响。这是否能提高克隆效率还有待进一步研究。
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