Sakamoto H, Atsumi S
Department of Anatomy, Yamanashi Medical College, Japan.
Cell Tissue Res. 1991 May;264(2):221-30. doi: 10.1007/BF00313959.
Substance P (SP)- and 5-hydroxytryptamine (5-HT)-containing presynaptic boutons in the cervical ventral horn were studied in chicken, hamster, rat and monkey spinal cords, using PAP and protein A-gold double-labeling techniques in conjunction with monoclonal antibodies. In the chicken, the PAP method demonstrated that SP-immunoreactive boutons contained large spherical dense-cored vesicles (DCVs) whereas 5-HT-immunoreactive boutons displayed both elongated and spherical DCVs. Using the protein A-gold double-labeling technique, 10-nm gold particles for SP were localized over the spherical DCV-containing boutons whereas 15-nm gold particles for 5-HT were localized on elongated DCV-containing boutons. On the other hand, in the other species investigated, both SP- and 5-HT-immunoreactive boutons had similar morphological features as shown by the PAP method; both contained elongated and spherical DCVs. The two different sized gold particles, each of which labeled either 5-HT or SP, were found together over DCVs in a single bouton. These results indicate that 5-HT and SP are contained in different presynaptic boutons in the chicken, although in the hamster, rat and Japanese macaque, the two neurotransmitters/modulators coexist in the same DCVs in a single bouton. Species differences have thus been demonstrated for the coexistence of 5-HT and SP in the spinal ventral horn.
运用过氧化物酶抗过氧化物酶（PAP）和蛋白A-金双重标记技术，结合单克隆抗体，对鸡、仓鼠、大鼠和猴脊髓颈段腹角中含P物质（SP）和5-羟色胺（5-HT）的突触前终末进行了研究。在鸡中，PAP法显示，含SP免疫反应性的终末含有大的球形致密核心囊泡（DCV），而含5-HT免疫反应性的终末则同时显示出细长形和球形的DCV。使用蛋白A-金双重标记技术，用于标记SP的10纳米金颗粒定位于含球形DCV的终末上，而用于标记5-HT的15纳米金颗粒则定位于含细长形DCV的终末上。另一方面，在所研究的其他物种中，PAP法显示含SP和含5-HT免疫反应性的终末具有相似的形态特征；两者均含有细长形和球形的DCV。在单个终末的DCV上同时发现了两种不同大小、分别标记5-HT或SP的金颗粒。这些结果表明，在鸡中5-HT和SP存在于不同的突触前终末，而在仓鼠、大鼠和日本猕猴中，这两种神经递质/调质共存于单个终末的同一DCV中。因此，已证明5-HT和SP在脊髓腹角中共存存在物种差异。
