Stefanová I, Horejsí V
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
J Immunol. 1991 Sep 1;147(5):1587-92.
mAb against human glycosyl-phosphatidylinositol-linked leucocyte surface Ag CD59 and CD55 immunoprecipitated from detergent lysates of HPB ALL cell line in addition to the respective Ag a common 80-kDa glycoprotein component and (glyco)lipids. The 80-kDa glycoprotein is different from otherwise similar CD44 Ag. The CD59 immunoprecipitate contained also a small amount of the CD55 glycoprotein and the CD55 immunoprecipitate minute amount of the CD59 Ag. These results are interpreted in terms of existence of noncovalent complexes resistant to dissociation by mild detergents and consisting of the 80-kDa glycoprotein, CD59 and CD55 glycoproteins, relatively tightly bound (glyco)lipids and possibly other so far unidentified components. These complexes contain probably also other glycosyl-phosphatidylinositol-linked Ag, as an anti-CD48 mAb immunoprecipitated also an apparently very similar complex. The complexes immunoprecipitated by mAb against the CD55, CD59, and CD48 Ag also contain a protein kinase activity. This type of complexes could not be demonstrated in several other cell types such as RBC, PBMC, and HeLa cells. However, a qualitatively very similar set of components was immunoprecipitated from the murine thymoma EL-4 cell line by an anti-Thy-1 mAb.
针对人糖基磷脂酰肌醇连接的白细胞表面抗原CD59和CD55的单克隆抗体，除了各自的抗原外，还从HPB ALL细胞系的去污剂裂解物中免疫沉淀出一种常见的80 kDa糖蛋白成分和（糖）脂。该80 kDa糖蛋白与其他方面类似的CD44抗原不同。CD59免疫沉淀物中还含有少量的CD55糖蛋白，而CD55免疫沉淀物中含有微量的CD59抗原。这些结果被解释为存在对温和去污剂解离有抗性的非共价复合物，该复合物由80 kDa糖蛋白、CD59和CD55糖蛋白、相对紧密结合的（糖）脂以及可能其他迄今未鉴定的成分组成。这些复合物可能还含有其他糖基磷脂酰肌醇连接的抗原，因为抗CD48单克隆抗体也免疫沉淀出一种明显非常相似的复合物。针对CD55、CD59和CD48抗原的单克隆抗体免疫沉淀的复合物也含有蛋白激酶活性。在其他几种细胞类型如红细胞、外周血单个核细胞和HeLa细胞中未发现这种类型的复合物。然而，抗Thy-1单克隆抗体从鼠胸腺瘤EL-4细胞系中免疫沉淀出一组性质非常相似的成分。
