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[一种修正被动血凝抑制试验以校正破伤风抗毒素滴定中被动血凝试验结果的方法]

[A modification of the passive hemagglutination inhibition test to correct the results of the passive hemagglutination test in titrating tetanus antitoxin].

作者信息

Stovbun S F, Porchesku L S

出版信息

Lab Delo. 1991(4):65-8.

PMID:1715439
Abstract

The aim of this work was to accelerate passive hemagglutination inhibition test, simplify it, perform it without an incubator and antibody diagnostic agent, and confirm the results of this test the characteristics permitting not only specific, but quantitative assessment of the results of passive hemagglutination test. To achieve this, the authors suggest the following modifications: the working dose of purified tetanus antitoxin is titered in relation to the standard serum; antibody erythrocytic diagnostic agent is modified by adding the working dose of purified tetanus antitoxin; the test is performed at room temperature.

摘要

这项工作的目的是加快被动血凝抑制试验,简化该试验,在无需培养箱和抗体诊断剂的情况下进行该试验,并确认该试验的结果,这些特性不仅允许对被动血凝试验的结果进行特异性评估,还能进行定量评估。为实现这一目标,作者提出以下改进方法:根据标准血清对纯化破伤风抗毒素的工作剂量进行滴定;通过添加纯化破伤风抗毒素的工作剂量来改良抗体红细胞诊断剂;在室温下进行试验。

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