Tian Xin, Bi Li-qi, Li Zhan-guo
Department of Pediatrics, Second Hospital of Jilin University, Changchun 130041, China.
Zhonghua Yi Xue Za Zhi. 2006 Sep 5;86(33):2334-8.
To investigate the significance of citrullinated collagen type II and its antibodies in the pathogenesis and diagnosis of rheumatoid arthritis (RA).
Serum samples were obtained from 175 patients with RA, 112 patients with systemic lupus erythematosus (SLE), 37 patients with osteoarthritis (OA), and 160 healthy controls. Arginine residues of bovine collagen type II (bCII) were converted to citrulline residues by peptidylarginine deiminase (PAD). The presence of antibodies against citullinated bCII (Cit-bCII) and bCII, a substrate protein of PAD, were examined by using enzyme-linked immunosorbent assay (ELISA) and Western blotting. The levels of anti-cyclic citrullinated peptide (CCP) antibodies, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and rheumatoid factor (RF) in the above patients were detected.
The positive rate of anti-CitbCII antibodies of the RA patients was 50.3% (88/175), significantly higher than those of the SLE patients (17.9%, 20/112), OA patients (18.9%, 7/37), and healthy controls (2.5%, 4/160). The positive rate of anti-b of the RA patients was 33.7% (59/175), significantly lower than that of the CII antibodies. The levels of anti-CitbCII and anti-bCII antibodies were not statistically different from SLE and OA patients. There was a close correlation between anti-CitbCII and anti-CCP antibodies. Spearman correlation analysis showed that anti-CitbCII antibodies were positively correlated with anti-CCP antibodies, and CRP, ESR, RF, X ray imaging, course of disease, and age were not associated with anti-CitbCII antibodies.
Anti-citrullinated bCII antibody is relatively RA-specific. Citrullination appears to be important in RA.
探讨瓜氨酸化II型胶原及其抗体在类风湿关节炎（RA）发病机制及诊断中的意义。
收集175例RA患者、112例系统性红斑狼疮（SLE）患者、37例骨关节炎（OA）患者及160例健康对照者的血清样本。用肽基精氨酸脱亚氨酶（PAD）将牛II型胶原（bCII）的精氨酸残基转化为瓜氨酸残基。采用酶联免疫吸附测定（ELISA）和蛋白质印迹法检测抗瓜氨酸化bCII（Cit-bCII）抗体及PAD底物蛋白bCII抗体的存在情况。检测上述患者的抗环瓜氨酸肽（CCP）抗体、C反应蛋白（CRP）、红细胞沉降率（ESR）及类风湿因子（RF）水平。
RA患者抗CitbCII抗体阳性率为50.3%（88/175），显著高于SLE患者（17.9%，20/112）、OA患者（18.9%，7/37）及健康对照者（2.5%，4/160）。RA患者抗bCII抗体阳性率为33.7%（59/175），显著低于抗CII抗体阳性率。抗CitbCII和抗bCII抗体水平在SLE和OA患者中无统计学差异。抗CitbCII与抗CCP抗体密切相关。Spearman相关分析显示，抗CitbCII抗体与抗CCP抗体呈正相关，且CRP、ESR、RF、X线影像、病程及年龄与抗CitbCII抗体无关。
抗瓜氨酸化bCII抗体相对具有RA特异性。瓜氨酸化在RA中似乎很重要。
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