Bischoff S C, Baggiolini M, de Weck A L, Dahinden C A
Institute of Clinical Immunology, Inselspital, Bern, Switzerland.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):628-33. doi: 10.1016/0006-291x(91)91418-c.
We have shown previously that interleukin 8 (IL-8) induces histamine and leukotriene release in human basophils exposed to interleukin 3 (IL-3). We now found that pretreatment with low concentrations of IL-8 selectively inhibits this response. Inhibition was significant at 0.01 nM IL-8 and virtually complete at 1 nM, which is about 100-fold lower than the concentration required for induction of mediator release. IL-8 dependent responses were also inhibited, albeit to a lesser extent, by preincubation with neutrophil-activating peptide 2 (NAP-2), but not with connective tissue-activating peptide III (CTAP-III) or platelet factor 4 (PF4). Release induced by C5a, fMet-Leu-Phe, or anti-IgE antibody, by contrast, was not affected.
我们之前已经表明，白细胞介素8（IL-8）可诱导暴露于白细胞介素3（IL-3）的人嗜碱性粒细胞释放组胺和白三烯。我们现在发现，用低浓度的IL-8预处理可选择性抑制这种反应。在0.01 nM IL-8时抑制作用显著，在1 nM时几乎完全抑制，这比诱导介质释放所需的浓度低约100倍。与中性粒细胞激活肽2（NAP-2）预孵育也可抑制IL-8依赖性反应，尽管程度较小，但与结缔组织激活肽III（CTAP-III）或血小板因子4（PF4）预孵育则无此作用。相比之下，由C5a、fMet-Leu-Phe或抗IgE抗体诱导的释放不受影响。
