Lin H M, Yen F L, Ng L T, Lin C C
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
J Ethnopharmacol. 2007 Apr 20;111(1):129-36. doi: 10.1016/j.jep.2006.11.004. Epub 2006 Nov 10.
Nuzhenzi, the fruit of Ligustrum lucidum Ait. (Oleaceae), is commonly used as tonic for kidney and liver in the traditional Chinese medicine prescription. The present study aimed to investigate the antioxidant activities of ethanol extract of Ligustrum lucidum fruits (ELL) and its effects on butylated hydroxytoluene (BHT)-induced oxidative stress in rats. Results showed that ELL possesses weak antioxidant activities. Compared to the BHT (1000mg/kg)-treated group, results showed that ELL at 250, 500 and 1000mg/kg significantly reduced the levels of blood urea nitrogen (BUN), serum glutamic pyruvic transaminase (sGPT), glutamic oxaloacetic transaminase (sGOT), alkaline phosphatase (sALP), lactate dehydrogenase (LDH), triglyceride (TG) and creatinine (Cr), as well as LDH in bronchoalveolar lavage fluid (BALF). It also significantly decreased the level of lipid peroxides in liver and lung. In addition, ELL significantly enhanced the levels of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in these organs. Histopathological evaluation of the tissues revealed that ELL reduced the incidence of lung lesions, while the liver and kidney tissues were not affected by BHT administration. Taken together, the protective effect of ELL against acute BHT-induced oxidative stress in rats could be through the upregulation of antioxidant enzymes.
女贞子为木犀科植物女贞（Ligustrum lucidum Ait.）的果实，在中药方剂中常用于滋补肝肾。本研究旨在探讨女贞果实乙醇提取物（ELL）的抗氧化活性及其对丁基羟基甲苯（BHT）诱导的大鼠氧化应激的影响。结果表明，ELL具有较弱的抗氧化活性。与BHT（1000mg/kg）处理组相比，结果显示250、500和1000mg/kg的ELL显著降低了血尿素氮（BUN）、血清谷丙转氨酶（sGPT）、谷草转氨酶（sGOT）、碱性磷酸酶（sALP）、乳酸脱氢酶（LDH）、甘油三酯（TG）和肌酐（Cr）水平，以及支气管肺泡灌洗液（BALF）中的LDH水平。它还显著降低了肝脏和肺中的脂质过氧化物水平。此外，ELL显著提高了这些器官中过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的水平。组织的组织病理学评估显示，ELL降低了肺部病变的发生率，而肝脏和肾脏组织未受BHT给药的影响。综上所述，ELL对大鼠急性BHT诱导的氧化应激的保护作用可能是通过上调抗氧化酶实现的。
