Li X M, Ma Y L, Liu X J
School of Food Science and Technology of the XingJiang Agriculture University, Urumqili City, XinJiang 830000, PR China.
J Ethnopharmacol. 2007 May 22;111(3):504-11. doi: 10.1016/j.jep.2006.12.024. Epub 2006 Dec 28.
Oxidative damage of biomolecules increases with age and is postulated to be a major causal factor of various physiological function disorders. Consequently, the concept of anti-age by antioxidants has been developed. Lycium barbarum fruits have been used as a traditional Chinese herbal medicine and the data obtained in in vitro models have clearly established the antioxidant potency of the polysaccharides isolated from the fruits. In the present study, the age-dependent changes in the antioxidant enzyme activity, immune function and lipid peroxidation product were investigated and effect of Lycium barbarum polysaccharides on age-induced oxidative stress in different organs of aged mice was checked. Lycium barbarum polysaccharides (200, 350 and 500 mg/kg b.w. in physiological saline) were orally administrated to aged mice over a period of 30 days. Aged mice receiving vitamin C served as positive control. Enzymatic and non-enzymatic antioxidants, lipid peroxides in serum and tested organs, and immune function were measured. Result showed that increased endogenous lipid peroxidation, and decreased antioxidant activities, as assessed by superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC), and immune function were observed in aged mice and restored to normal levels in the polysaccharides-treated groups. Antioxidant activities of Lycium barbarum polysaccharides can be compable with normal antioxidant, vitamin C. Moreover, addition of vitamin C to the polysaccharides further increased the in vivo antioxidant activity of the latter. It is concluded that the Lycium barbarum polysaccharides can be used in compensating the decline in TAOC, immune function and the activities of antioxidant enzymes and thereby reduces the risks of lipid peroxidation accelerated by age-induced free radical.
生物分子的氧化损伤随年龄增长而增加，据推测是各种生理功能紊乱的主要致病因素。因此，抗氧化剂抗老化的概念应运而生。枸杞果实一直被用作传统中药，体外模型实验数据已明确证实从枸杞果实中分离出的多糖具有抗氧化能力。在本研究中，研究了抗氧化酶活性、免疫功能和脂质过氧化产物随年龄的变化，并检测了枸杞多糖对老年小鼠不同器官中年龄诱导的氧化应激的影响。将枸杞多糖（在生理盐水中分别为200、350和500mg/kg体重）给老年小鼠连续口服30天。接受维生素C的老年小鼠作为阳性对照。检测了血清和受试器官中的酶促和非酶促抗氧化剂、脂质过氧化物以及免疫功能。结果显示，老年小鼠体内内源性脂质过氧化增加，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化能力（TAOC）评估的抗氧化活性降低，免疫功能下降，而多糖治疗组恢复到正常水平。枸杞多糖的抗氧化活性可与正常抗氧化剂维生素C相媲美。此外，在多糖中添加维生素C进一步提高了其体内抗氧化活性。得出结论，枸杞多糖可用于弥补TAOC、免疫功能和抗氧化酶活性的下降，从而降低年龄诱导的自由基加速脂质过氧化的风险。
