Thomas Mariyamma, Sujatha K S, George Sisilamma
Department of Veterinary Biochemistry, Faculty of Veterinary and Animal Sciences, Mannuthy, Thrissur 680 651, India.
Indian J Exp Biol. 2009 Mar;47(3):186-92.
Protective effect of ethanol extract of Piper longum Linn. against monosodium glutamate (MSG) induced toxicity was studied. Rats, orally administered with MSG at a dose of 8 mg/g body weight for 20 consecutive days, showed an increase in liver weight and rate of lipid peroxidation. Glutathione (GSH) in serum, liver and kidney showed decreased concentration. Significant increase was noticed in activities of serum alanine amino transferase (ALT) and aspartate amino transferase (AST), levels of serum triacylglycerol, total cholesterol and urea. Histopathological examination of liver and kidney showed central venous congestion, diffuse degeneration and necrosis of hepatocytes in para cortical and midzonal areas of liver and diffuse cortical tubular degeneration of kidney. Oral administration of ethanol extract of P. longum fruits at 300 mg/kg body weight along with MSG significantly reduced the levels of lipid peroxides in serum, liver and kidney, serum AST activity, serum levels of triacylglycerol and total cholesterol. Though, there was an increase in the level of GSH in tissues it was not significant. However, the treatment failed to reduce the levels of ALT and urea. Examination of tissue sections also exhibited normal histological architecture of both the organs. The present study revealed that administration of P. longum provided significant protection to liver and kidney from the oxidative stress of MSG, though the dose rate was not sufficient to provide a complete protection.
研究了荜茇乙醇提取物对味精（MSG）诱导的毒性的保护作用。大鼠连续20天口服8mg/g体重的味精，肝脏重量增加，脂质过氧化速率升高。血清、肝脏和肾脏中的谷胱甘肽（GSH）浓度降低。血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性、血清三酰甘油、总胆固醇和尿素水平显著升高。肝脏和肾脏的组织病理学检查显示，肝脏中央静脉淤血，肝小叶旁皮质和中区肝细胞弥漫性变性和坏死，肾脏皮质肾小管弥漫性变性。以300mg/kg体重的荜茇果实乙醇提取物与味精一起口服，可显著降低血清、肝脏和肾脏中的脂质过氧化物水平、血清AST活性、血清三酰甘油和总胆固醇水平。虽然组织中GSH水平有所升高，但不显著。然而，该治疗未能降低ALT和尿素水平。组织切片检查也显示两个器官的组织学结构正常。本研究表明，荜茇给药对肝脏和肾脏免受味精的氧化应激有显著保护作用，尽管剂量率不足以提供完全保护。
