Wagner D K, Wong H L, Gately M K, Nelson D L
Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Cytokine. 1990 Sep;2(5):337-43. doi: 10.1016/1043-4666(90)90063-y.
Previous studies have shown that peripheral blood mononuclear cells activated in vitro not only express cell-associated interleukin 2 receptors (IL2R) but also release a soluble form of this receptor. In this study, we demonstrate that administration of human recombinant IL 2 (rIL 2) to mice results in increased spleen weights, splenic natural killer (NK) cell cytolytic activity, and serum levels of soluble IL2R. However, compared with rIL 2-treated heterozygote controls, beige mice treated with rIL 2 displayed similar elevations in serum soluble IL2R but significantly less splenic NK activity. Likewise, administration of anti-asialo GM1 antiserum to rIL 2-treated mice resulted in a dramatic reduction in splenic NK cytolytic activity, but no reduction in serum soluble IL2R. Conversely, while rIL 2 treatment of BALB/c mice produced increased splenic NK activity and serum soluble IL2R, similar treatment of BALB/c nude mice resulted in elevation of only splenic NK activity. These studies demonstrate that administration of rIL 2 to normal mice can elevate both serum IL2R levels and splenic NK cytolytic activity. However, the results suggest that T cells are likely to be the source of elevated serum IL2R after rIL 2 administration.
以往的研究表明，体外激活的外周血单个核细胞不仅表达细胞相关的白细胞介素2受体（IL2R），还释放该受体的可溶性形式。在本研究中，我们证明给小鼠注射人重组白细胞介素2（rIL 2）会导致脾脏重量增加、脾脏自然杀伤（NK）细胞的细胞溶解活性增强以及血清可溶性IL2R水平升高。然而，与用rIL 2处理的杂合子对照相比，用rIL 2处理的米色小鼠血清可溶性IL2R升高程度相似，但脾脏NK活性明显较低。同样，给用rIL 2处理的小鼠注射抗唾液酸GM1抗血清会导致脾脏NK细胞溶解活性显著降低，但血清可溶性IL2R没有降低。相反，虽然用rIL 2处理BALB/c小鼠会使脾脏NK活性和血清可溶性IL2R增加，但对BALB/c裸鼠进行类似处理仅导致脾脏NK活性升高。这些研究表明，给正常小鼠注射rIL 2可提高血清IL2R水平和脾脏NK细胞溶解活性。然而，结果表明T细胞可能是注射rIL 2后血清IL2R升高的来源。
