Malkas Linda H, Herbert Brittney Shea, Abdel-Aziz Waleed, Dobrolecki Lacey E, Liu Yang, Agarwal Beamon, Hoelz Derek, Badve Sunil, Schnaper Lauren, Arnold Randy J, Mechref Yehia, Novotny Milos V, Loehrer Patrick, Goulet Robert J, Hickey Robert J
Division of Hematology and Oncology, Indiana University School of Medicine, and Indiana Cancer Center, Indianapolis, IN 46202, USA.
Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19472-7. doi: 10.1073/pnas.0604614103. Epub 2006 Dec 11.
Two isoforms of proliferating cell nuclear antigen (PCNA) have been observed in breast cancer cells. Commercially available antibodies to PCNA recognize both isoforms and, therefore, cannot differentiate between the PCNA isoforms in malignant and nonmalignant breast epithelial cells and tissues. We have developed a unique antibody that specifically detects a PCNA isoform (caPCNA) associated with breast cancer epithelial cells grown in culture and breast-tumor tissues. Immunostaining studies using this antibody suggest that the caPCNA isoform may be useful as a marker of breast cancer and that the caPCNA-specific antibody could potentially serve as a highly effective detector of malignancy. We also report here that the caPCNA isoform functions in breast cancer-cell DNA replication and interacts with DNA polymerase delta. Our studies indicate that the caPCNA isoform may be a previously uncharacterized detector of breast cancer.
在乳腺癌细胞中观察到了增殖细胞核抗原（PCNA）的两种亚型。市售的PCNA抗体可识别这两种亚型，因此，无法区分恶性和非恶性乳腺上皮细胞及组织中的PCNA亚型。我们开发了一种独特的抗体，该抗体能特异性检测与培养的乳腺癌上皮细胞及乳腺肿瘤组织相关的一种PCNA亚型（caPCNA）。使用该抗体进行的免疫染色研究表明，caPCNA亚型可能作为乳腺癌的标志物，并且caPCNA特异性抗体可能潜在地作为一种高效的恶性肿瘤检测器。我们在此还报告，caPCNA亚型在乳腺癌细胞DNA复制中发挥作用，并与DNA聚合酶δ相互作用。我们的研究表明，caPCNA亚型可能是一种此前未被鉴定的乳腺癌检测器。
