Takeda Kazuhiko, Katoh Shinya, Nakatani Nobutake, Sakugawa Hiroshi
Graduate School of Biosphere Science, Hiroshima University, Japan.
Anal Sci. 2006 Dec;22(12):1509-14. doi: 10.2116/analsci.22.1509.
The aim of this research was to develop a simple procedure for a highly sensitive determination of low-molecular-weight (LMW) carbonyl compounds in drinking water and natural water. We employed a preconcentration HPLC system with 2,4-dinitrophenylhydrazine (DNPH) for the determination of LMW carbonyl compounds. A C-18 reverse-phase preconcentration column was used instead of a sample loop at the sample injection valve. A 0.1 - 5.0 mL portion of the derivatized sample solution was injected with a gas-tight syringe, and a 15% acetonitrile aqueous solution was pushed through the preconcentration column to remove the unreacted excess DNPH, which caused serious interference in the determination of formaldehyde. The detection limits were 1 - 3 nM with a relative standard deviation of 2 - 5% for 20 nM standard solutions (n = 5). The calibration curves were essentially unaffected by coexisting sea salts. Applications to commercial mineral water, tap water, river water, pond water and seawater are presented.
本研究的目的是开发一种简单的程序，用于高灵敏度测定饮用水和天然水中的低分子量（LMW）羰基化合物。我们采用了带有2,4-二硝基苯肼（DNPH）的预浓缩HPLC系统来测定LMW羰基化合物。在进样阀处使用C-18反相预浓缩柱代替样品定量环。用气密注射器注入0.1 - 5.0 mL衍生化样品溶液，然后将15%的乙腈水溶液推过预浓缩柱，以去除未反应的过量DNPH，其会对甲醛的测定造成严重干扰。对于20 nM的标准溶液（n = 5），检测限为1 - 3 nM，相对标准偏差为2 - 5%。校准曲线基本不受共存海盐的影响。文中还介绍了该方法在市售矿泉水、自来水、河水、池塘水和海水中的应用。
