Sandner F, Dott W, Hollender J
Institut für Hygiene und Umweltmedizin, RWTH Aachen, Pauwelsstrasse 30, D-52074 Aachen, Germany.
Int J Hyg Environ Health. 2001 Mar;203(3):275-9. doi: 10.1078/s1438-4639(04)70038-3.
The toxic potential of formaldehyde and other aliphatic/aromatic carbonyl compounds requires the determination of even low amounts of these compounds in indoor air. The existing DFG-method for workplace monitoring using adsorption at 2,4-dinitrophenylhydrazine (DNPH)-coated sorbents followed by HPLC-UV/DAD analysis of the extract was modified in order to decrease detection limits. The improvement included an increase in volume and rate of the air sampling, testing applicability of different adsorption materials and a decrease of the extraction volume of the hydrazones. 13 DNPH-derivatives could be separated well on a RP18-column followed by UV/DAD-detection at 365 nm. Recovery rates of 70-100% were determined (apart from acetone with 19%) using dynamically produced artifical carbonyl atmospheres. Detection limits of 0.05-0.4 microgram/m3 were reached by this method which are sufficient for indoor air monitoring.
甲醛及其他脂肪族/芳香族羰基化合物的潜在毒性要求测定室内空气中痕量的这些化合物。现有的德国研究联合会（DFG）方法用于工作场所监测，该方法是用涂有2,4-二硝基苯肼（DNPH）的吸附剂进行吸附，然后对提取物进行高效液相色谱-紫外/二极管阵列检测（HPLC-UV/DAD），为降低检测限对该方法进行了改进。改进措施包括增加空气采样的体积和速率、测试不同吸附材料的适用性以及减少腙的萃取体积。13种DNPH衍生物在RP18柱上能很好地分离，随后在365nm处进行紫外/二极管阵列检测。使用动态生成的人工羰基气氛测定回收率为70%-100%（丙酮除外，其回收率为19%）。该方法的检测限达到0.05-0.4微克/立方米，足以用于室内空气监测。
