Kiselev Iu L, Tsittser A O
Lab Delo. 1991(5):59-60.
The activity of purified V. cholerae cytolysin was estimated from its cytotoxic effect on L-929 cells in various growth phases. The period of target cell development was supposed to influence the sensitivity of the test. Cytolysin preparation is thermolabile, and its effect is neutralized with homologous antiserum.
通过纯化的霍乱弧菌溶细胞素对处于不同生长阶段的L-929细胞的细胞毒性作用来评估其活性。靶细胞的发育时期被认为会影响检测的敏感性。溶细胞素制剂不耐热,其作用可被同源抗血清中和。