肝细胞中的肌球蛋白对于胆小管收缩至关重要。

Myosin in hepatocytes is essential for bile canalicular contraction.

作者信息

Watanabe S, Miyazaki A, Hirose M, Takeuchi M, Ohide H, Kitamura T, Ueno T, Kominami E, Sato N

机构信息

Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.

出版信息

Liver. 1991 Jun;11(3):185-9. doi: 10.1111/j.1600-0676.1991.tb00513.x.

DOI:10.1111/j.1600-0676.1991.tb00513.x

PMID:1715965

Abstract

Active dynamic contraction of bile canaliculi has been observed in cultured doublet hepatocytes using time-lapse cinephotomicrography. This contractile movement plays an important role in normal bile formation. The mechanism of bile canalicular contraction has been proved to involve the Ca(2+)-calmodulin system and pericanalicular actin filaments. However, the role of myosin in this system is still unknown. In this study, using the newly synthesized myosin light-chain kinase inhibitor ML-9, we found that the treatment of cultured doublet hepatocytes with ML-9 inhibited canalicular contraction. This inhibitory effect suggests that myosin is involved in this complex cellular function and that the integrity of the actin-myosin system, as well as the Ca(2+)-calmodulin system is essential for normal bile canalicular contraction.

摘要

利用延时电影显微镜技术，在培养的双联体肝细胞中观察到胆小管的主动动态收缩。这种收缩运动在正常胆汁形成过程中发挥着重要作用。已证明胆小管收缩机制涉及Ca(2+)-钙调蛋白系统和胆小管周围肌动蛋白丝。然而，肌球蛋白在该系统中的作用仍不清楚。在本研究中，使用新合成的肌球蛋白轻链激酶抑制剂ML-9，我们发现用ML-9处理培养的双联体肝细胞可抑制胆小管收缩。这种抑制作用表明肌球蛋白参与了这种复杂的细胞功能，并且肌动蛋白-肌球蛋白系统以及Ca(2+)-钙调蛋白系统的完整性对于正常的胆小管收缩至关重要。