Watanabe S, Hirose M, Ueno T, Kominami E, Namihisa T
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
Liver. 1990 Aug;10(4):249-54. doi: 10.1111/j.1600-0676.1990.tb00466.x.
The mechanism of phagocytosis by Kupffer cells is believed to involve the Ca2(+)-calmodulin system. However, the role of myosin in this system is still unknown. In this study, we found that myosin light chain kinase inhibitor (ML-9) inhibited phagocytosis by cultured Kupffer cells using polystyrene beads, a time-lapse VTR system and fluorescent staining techniques. The inhibitory effects of ML-9 suggest that myosin may be involved in this complex cellular function and that the integrity of the cytoskeletal system is essential for normal phagocytosis.
据信，库普弗细胞的吞噬机制涉及Ca2(+)-钙调蛋白系统。然而，肌球蛋白在该系统中的作用仍不清楚。在本研究中，我们发现肌球蛋白轻链激酶抑制剂（ML-9）使用聚苯乙烯珠、延时录像系统和荧光染色技术抑制培养的库普弗细胞的吞噬作用。ML-9的抑制作用表明肌球蛋白可能参与了这种复杂的细胞功能，并且细胞骨架系统的完整性对于正常吞噬作用至关重要。
