Martineau B, McBride K E, Houck C M
Calgene, Inc., Davis, CA 95616.
Mol Gen Genet. 1991 Aug;228(1-2):281-6. doi: 10.1007/BF00282477.
Tomato fruits contain a metallocarboxypeptidase inhibitor (MCPI) the sequence of which has already been determined. Here we report the isolation of a tomato cDNA clone that encodes the mature MCPI protein as well as an N-terminal signal peptide for entry into the secretory system and an eight amino acid carboxyterminal extension. MCPI RNA is present at very high levels in anthesis stage ovaries and decreases quite rapidly during fruit development. MCPI protein accumulation reflects the pattern of MCPI RNA accumulation in fruit, consistent with a transcriptional control of MCPI gene activity. In leaves, the levels of MCPI RNA and protein are very low. Wounding of the leaves causes a dramatic (100-fold) increase in steady-state level of MCPI RNA without a concomitant increase in MCPI protein level suggesting a control at the post-transcriptional or translational level of gene expression. Genomic DNA blot hybridization data indicate that MCPI in tomato may be encoded by a single gene.
番茄果实含有一种金属羧肽酶抑制剂（MCPI），其序列已经确定。在此，我们报告了一个番茄cDNA克隆的分离，该克隆编码成熟的MCPI蛋白以及用于进入分泌系统的N端信号肽和一个八氨基酸的羧基末端延伸。MCPI RNA在开花期卵巢中含量非常高，在果实发育过程中迅速下降。MCPI蛋白积累反映了果实中MCPI RNA积累的模式，这与MCPI基因活性的转录控制一致。在叶片中，MCPI RNA和蛋白的水平非常低。叶片受伤会导致MCPI RNA的稳态水平急剧（100倍）增加，而MCPI蛋白水平没有相应增加，这表明在基因表达的转录后或翻译水平上存在调控。基因组DNA印迹杂交数据表明，番茄中的MCPI可能由单个基因编码。
