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猕猴桃(美味猕猴桃变种美味猕猴桃)果实发育过程中差异表达的五个基因的cDNA克隆与特性分析

Cloning and characterization of five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var. deliciosa).

作者信息

Ledger S E, Gardner R C

机构信息

Centre for Gene Technology, School of Biological Sciences, University of Auckland, New Zealand.

出版信息

Plant Mol Biol. 1994 Aug;25(5):877-86. doi: 10.1007/BF00028882.

Abstract

Five cDNAs for genes differentially expressed during fruit development of kiwifruit (Actinidia deliciosa var. deliciosa cv. Hayward) were isolated from a library made from young fruit, 8-10 days after anthesis. One gene (pKIWI503) has low levels of expression in young fruit but is induced late in fruit development and during fruit ripening, and has some homology to plant metallothionein-like proteins. The other four genes are highly expressed in young fruit with reduced expression in the later stages of fruit development. pKIWI504 has strong homology to plant metallothionein-like proteins and pKIWI505 exhibits homology to the beta-subunit of the mitochondrial ATP synthase gene. The two other genes (pKIWI501 and 502) encode proteins with no significant homology to other known sequences.

摘要

从花期后8 - 10天的猕猴桃(美味猕猴桃品种海沃德)幼果构建的文库中,分离出了5个在果实发育过程中差异表达基因的cDNA。其中一个基因(pKIWI503)在幼果中表达水平较低,但在果实发育后期和成熟过程中被诱导表达,并且与植物类金属硫蛋白有一定同源性。另外四个基因在幼果中高表达,而在果实发育后期表达降低。pKIWI504与植物类金属硫蛋白有很强的同源性,pKIWI505与线粒体ATP合酶基因的β亚基具有同源性。另外两个基因(pKIWI501和502)编码的蛋白质与其他已知序列没有明显的同源性。

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