用于重组大肠杆菌B生产乳酸和乙醇的低盐培养基。

Low salt medium for lactate and ethanol production by recombinant Escherichia coli B.

作者信息

Martinez Alfredo, Grabar T B, Shanmugam K T, Yomano L P, York S W, Ingram L O

机构信息

Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Morelos, Mexico.

出版信息

Biotechnol Lett. 2007 Mar;29(3):397-404. doi: 10.1007/s10529-006-9252-y. Epub 2006 Dec 12.

DOI:10.1007/s10529-006-9252-y

PMID:17160622

Abstract

Individual nutrient salts were experimentally varied to determine the minimum requirements for efficient L (+)-lactate production by recombinant strains of Escherichia coli B. Based on these results, AM1 medium was formulated with low levels of alkali metals (4.5 mM and total salts (4.2 g l(-1)). This medium was equally effective for ethanol production from xylose and lactate production from glucose with average productivities of 18-19 mmol l(-1) h(-1) for both (initial 48 h of fermentation).

摘要

通过实验改变单一营养盐，以确定大肠杆菌B重组菌株高效生产L（+）-乳酸的最低要求。基于这些结果，配制了碱金属含量低（4.5 mM）和总盐含量低（4.2 g l⁻¹）的AM1培养基。这种培养基对于从木糖生产乙醇和从葡萄糖生产乳酸同样有效，在两种情况下（发酵的初始48小时）平均生产率均为18 - 19 mmol l⁻¹ h⁻¹。