前列腺萎缩：缺氧诱导因子的免疫组织化学研究

Prostatic atrophy: immunohistochemical study of hypoxia induced factors.

作者信息

Meirelles Luciana R, Billis Athanase, Magna Luis A, Vassallo Jose, Matsura Marisa A

机构信息

Department of Anatomic Pathology, School of Medicine, State University of Campinas (UNICAMP), Mail Post 6111, Zip Code 13084971, Campinas, Brazil.

出版信息

Int Urol Nephrol. 2006;38(3-4):577-81. doi: 10.1007/s11255-006-6657-4.

DOI:10.1007/s11255-006-6657-4

PMID:17160630

Abstract

BACKGROUND

There is evidence that chronic local ischemia may be one possible etiology of prostatic atrophy (PA). Our aim was to study the expression of hypoxia induced factors in areas of PA.

DESIGN

The immunohistochemical expression of hypoxia-inducible factor-1 alpha (HIF-1) and vascular endothelial growth factor (VEGF) was studied in atrophic acini of 33 needle prostatic biopsies. Prostatic atrophy was the only diagnosis in these biopsies. For HIF-1alpha, a total of 27,158, 10,060 and 9920 nuclei were counted in atrophic acini, acini with squamous metaplasia, and acini without squamous metaplasia surrounding acute infarct, respectively. For VEGF, a total of 24,966, 9849 and 9918 nuclei were counted for the same acini. A total of 1326 nuclei were counted in acini of the needle biopsy used as negative control. The Student's test for independent samples with significance level at 5% (p<0.05) was used to compare the mean ratio (MR) of positive nuclei over total nuclei for HIF-1 and VEGF.

RESULTS

For HIF-1, 108 (MR=0.004), 6877 (MR=0.68), and 6566 (MR=0.66) nuclei were positive in atrophic acini, acini with squamous metaplasia, and acini without squamous metaplasia surrounding acute infarct, respectively. There was a highly significant difference (p<0.001) between atrophic acini and acini surrounding acute infarct. For VEGF, 139 (MR=0.05), 5926 (MR=0.60), and 4948 (MR=0.50) nuclei were positive in the same acini. The difference was highly significant (p<0.001). All 1326 nuclei counted in the needle biopsy with essentially normal findings were negative for HIF-1 and VEGF.

CONCLUSIONS

This study showed that prostatic atrophic acini are not on acute ischemia. However, local chronic ischemia cannot be ruled out. Experimental data showed that on chronic ischemia, the signal triggering HIF-1alpha accumulation may disappear despite continuous hypoxia suggesting that compensatory mechanisms triggered during prolonged hypoxia may be able to restore normal tissue oxygen levels.

摘要

背景

有证据表明慢性局部缺血可能是前列腺萎缩（PA）的一种可能病因。我们的目的是研究缺氧诱导因子在前列腺萎缩区域的表达。

设计

研究了33例前列腺穿刺活检标本中萎缩腺泡内缺氧诱导因子-1α（HIF-1）和血管内皮生长因子（VEGF）的免疫组化表达。这些活检标本的唯一诊断为前列腺萎缩。对于HIF-1α，分别在萎缩腺泡、伴有鳞状化生的腺泡以及急性梗死灶周围无鳞状化生的腺泡中计数了总共27158、10060和9920个细胞核。对于VEGF，对相同的腺泡计数了总共24966、9849和9918个细胞核。在用作阴性对照的穿刺活检腺泡中计数了总共1326个细胞核。采用显著性水平为5%（p<0.05）的独立样本t检验来比较HIF-1和VEGF阳性细胞核与总细胞核的平均比率（MR）。

结果

对于HIF-1，萎缩腺泡、伴有鳞状化生的腺泡以及急性梗死灶周围无鳞状化生的腺泡中分别有108个（MR = 0.004）、6877个（MR = 0.68）和6566个（MR = 0.66）细胞核呈阳性。萎缩腺泡与急性梗死灶周围的腺泡之间存在高度显著差异（p<0.001）。对于VEGF，相同腺泡中分别有139个（MR = 0.05）、5926个（MR = 0.60）和4948个（MR = 0.50）细胞核呈阳性。差异高度显著（p<0.001）。在基本正常的穿刺活检标本中计数的所有1326个细胞核HIF-1和VEGF均为阴性。