Brundrett M C, Kendrick B, Peterson C A
Soil Science and Plant Nutrition, University of Western Australia, Nedlands.
Biotech Histochem. 1991;66(3):111-6. doi: 10.3109/10520299109110562.
Polyethylene glycol (400) with 90% glycerol (aqueous) is introduced as an efficient solvent system for lipid stains. Various lipid-soluble dyes were dissolved in this solvent system and tested for their intensity, contrast, and specificity of staining of suberin lamellae in plant tissue. The stability (i.e., lack of precipitation) of the various staining solutions in the presence of fresh tissue was also tested. When dissolved in polyethylene glycol-glycerol, Sudan red 7B (fat red) was the best nonfluorescent stain and fluorol yellow 088 (solvent green 4) was an excellent fluorochrome. These two dyes formed stable staining solutions which efficiently stained lipids in fresh sections without forming precipitates. Estimations of the solubilities of these dyes in the solvent compared with their solubilities in lipids of various chemical types indicated that they should both be effective stains for lipids in general.
聚乙二醇（400）与90%甘油（水溶液）被引入作为一种用于脂质染色的高效溶剂体系。将各种脂溶性染料溶解在该溶剂体系中，并测试其对植物组织中栓质层的染色强度、对比度和特异性。还测试了各种染色溶液在新鲜组织存在下的稳定性（即无沉淀）。当溶解在聚乙二醇-甘油中时，苏丹红7B（脂肪红）是最佳的非荧光染料，而荧光黄088（溶剂绿4）是一种出色的荧光染料。这两种染料形成了稳定的染色溶液，能有效染色新鲜切片中的脂质而不形成沉淀。将这些染料在溶剂中的溶解度与其在各种化学类型脂质中的溶解度进行比较，结果表明它们总体上都应是有效的脂质染料。
