Nellaiappan K, Banu M J
Department of Zoology, University of Madras, India.
Biotech Histochem. 1991;66(3):125-30. doi: 10.3109/10520299109110565.
The improved method presented here for localizing monophenoloxidase activity of tyrosinase (E.C. 1.14.18.1) after electrophoresis is based on the transfer of electrons from the monophenolic substrate, tyrosine methyl ester, to an artificial acceptor, phenazine methosulfate, and subsequent reduction of nitro blue tetrazolium into a violet formazan. This method is rapid, sensitive and versatile compared to the standard method. The electron transferred from monophenol can be accepted directly by nitro blue tetrazolium; although the background of the gel is clear, the sensitivity is decreased. The monophenol-PMS-NBT method is suitable for both plant and animal samples. This method can also be used for histochemical demonstration of monophenoloxidase activity.
本文介绍的用于电泳后定位酪氨酸酶（E.C. 1.14.18.1）单酚氧化酶活性的改进方法，是基于电子从单酚底物酪氨酸甲酯转移至人工受体硫酸甲酯吩嗪，随后将硝基蓝四唑还原为紫色甲臜。与标准方法相比，该方法快速、灵敏且通用。从单酚转移的电子可直接被硝基蓝四唑接受；虽然凝胶背景清晰，但灵敏度会降低。单酚 - PMS - NBT方法适用于植物和动物样品。该方法也可用于单酚氧化酶活性的组织化学显示。
