肝移植中通过组织病理学和原位杂交诊断巨细胞病毒性肝炎

Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation.

作者信息

Espy M J, Paya C V, Holley K E, Ludwig J, Hermans P F, Wiesner R H, Krom R A, Smith T F

机构信息

Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.

出版信息

Diagn Microbiol Infect Dis. 1991 Jul-Aug;14(4):293-6. doi: 10.1016/0732-8893(91)90019-c.

DOI:10.1016/0732-8893(91)90019-c

PMID:1716192

Abstract

Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.

摘要

对巨细胞病毒（CMV）包涵体进行苏木精-伊红染色（H&E），并与使用生物素化DNA探针检测CMV的原位杂交进行比较。从23例临床诊断为CMV肝炎且组织病理学检查有典型CMV包涵体的患者中选取29份活检标本，其中23份（79%）经DNA探针检测为阳性，17份（59%）在细胞培养中检测到。与H&E染色（每个组织切片平均5个CMV病灶）相比，DNA探针检测到的每个组织切片中CMV病灶的平均数量更高（12个）。我们不建议微生物实验室将原位杂交作为组织标本中CMV诊断的组织病理学替代方法。