Gleaves C A, Hursh D A, Rice D H, Meyers J D
Diagnostic Virology Laboratory, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
J Clin Microbiol. 1989 Jan;27(1):21-3. doi: 10.1128/jcm.27.1.21-23.1989.
An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). However, the earliest that CMV could be detected by DNA hybridization was 58 h as compared with 16 h with monoclonal antibodies following centrifugation. DNA hybridization remains of great interest for the study and detection of CMV infection. However, current DNA hybridization techniques are not sufficiently rapid to replace the use of monoclonal antibodies in centrifugation culture.
评估了一种用于检测巨细胞病毒(CMV)的原位DNA杂交试剂盒在离心培养中检测CMV的能力。在61份临床标本中,17份(27.8%)在离心后经单克隆抗体染色显示CMV呈阳性。在这17份阳性标本中,15份通过DNA杂交检测到(24.5%)。然而,DNA杂交检测到CMV的最早时间为58小时,而离心后用单克隆抗体检测的最早时间为16小时。DNA杂交对于CMV感染的研究和检测仍然具有重要意义。然而,目前的DNA杂交技术不够快速,无法在离心培养中取代单克隆抗体的使用。