May E, Kopecka H, May P
Nucleic Acids Res. 1975 Oct;2(10):1995-2005. doi: 10.1093/nar/2.10.1995.
This paper describes the purification of polysomal RNA from SV40-lytically infected CV1 (monkey) cells and separation of the two distinct classes of SV40-specific mRNA sedimenting at 16 S and 19 S. These classes have been hybridized with the whole SV40 DNA genome as well as with the SV40 Hind fragments. The results have permitted the mapping of SV40-specific late 16 S mRNA from approximately 0.945 to 0.175 map units.
本文描述了从SV40裂解感染的CV1(猴)细胞中纯化多聚核糖体RNA,并分离出沉降系数为16S和19S的两类不同的SV40特异性mRNA。这些类别已与整个SV40 DNA基因组以及SV40 Hind片段进行杂交。结果使得能够将SV40特异性晚期16S mRNA定位在大约0.945至0.175图谱单位之间。