Weinberg R A, Warnaar S O, Winocour E
J Virol. 1972 Aug;10(2):193-201. doi: 10.1128/JVI.10.2.193-201.1972.
Deoxyribonucleic acid-ribonucleic acid (RNA) hybridization in formamide was used to isolate simian virus 40-specific RNA. Early in the lytic cycle, a 19S viral RNA species was observed. Late in the lytic cycle, 16S and 19S viral species were found. The 16S and 19S species of viral RNA were localized in the cytoplasm. High-molecular-weight heterogeneous RNA, containing viral sequences, was isolated from the nuclear fraction of infected cells late in the lytic cycle. This RNA may contain non-viral sequences linked to viral sequences. The formamide hybridization technique can be used to isolate intact late lytic viral RNA which is at least 99% pure.
利用甲酰胺中的脱氧核糖核酸-核糖核酸（RNA）杂交技术分离猴病毒40特异性RNA。在裂解周期早期，观察到一种19S病毒RNA种类。在裂解周期后期，发现了16S和19S病毒种类。病毒RNA的16S和19S种类定位于细胞质中。在裂解周期后期，从受感染细胞的核部分分离出含有病毒序列的高分子量异质性RNA。这种RNA可能含有与病毒序列相连的非病毒序列。甲酰胺杂交技术可用于分离纯度至少为99%的完整晚期裂解病毒RNA。
