Ribatti D, Nico B, Mangieri D, Longo V, Sansonno D, Vacca A, Dammacco F
Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.
Histol Histopathol. 2007 Mar;22(3):285-9. doi: 10.14670/HH-22.285.
This paper illustrates the use of the chick embryo chorioallantoic membrane (CAM) assay to determine the single and combined antiangiogenic effects of very low doses of vinblastine (VBL) and rapamycin (RAP) in human hepatocellular carcinoma (HCC). The angiogenic response induced by human HCC biopsy specimens was inhibited by each drug and sinergistically by their combination. Morever, immunohistochemical detection of microvessels with anti-CD31 mAB showed that their area was significantly lower in specimens treated with VBL and RAP in combination. Sinergy on the part of these well-known drugs when used in combination as antiangiogenics at very low doses may be of significance in the designing of new ways of treating HCC.
本文阐述了利用鸡胚绒毛尿囊膜（CAM）试验来测定极低剂量长春碱（VBL）和雷帕霉素（RAP）对人肝细胞癌（HCC）的单一及联合抗血管生成作用。每种药物均可抑制人肝癌活检标本诱导的血管生成反应，二者联合使用时具有协同作用。此外，用抗CD31单克隆抗体对微血管进行免疫组化检测显示，联合使用VBL和RAP处理的标本中微血管面积显著降低。这些知名药物在极低剂量联合用作抗血管生成剂时的协同作用，可能对设计治疗HCC的新方法具有重要意义。
