Du G, Fischer B, Barberet P, Heiss M
Gesellschaft für Schwerionenforschung GSI, Planckstrasse 1, 64291 Darmstadt, Germany.
Radiat Prot Dosimetry. 2006;122(1-4):320-2. doi: 10.1093/rpd/ncl435. Epub 2006 Dec 12.
For a single ion hit facility built to irradiate specific targets inside biological cells, it is necessary to prove that the ions hit the selected targets reliably because the ion hits usually cannot be seen. That ability is traditionally tested either indirectly by aiming at pre-etched tracks in a nuclear track detector or directly by making the ion tracks inside cells visible using a stain coupled to special proteins produced in response to ion hits. However, both methods are time consuming and hits can be verified only after the experiment. This means that targeting errors in the experiment cannot be corrected during the experiment. Therefore, we have developed a fast online hit verification method that measures the targeting accuracy electronically with a spatial resolution of +/-1 microm before cell irradiation takes place.
对于为照射生物细胞内特定靶点而建造的单离子撞击设备,由于离子撞击通常不可见,因此有必要证明离子能可靠地撞击所选靶点。传统上,这种能力要么通过瞄准核径迹探测器中的预蚀刻径迹进行间接测试,要么通过使用与响应离子撞击而产生的特殊蛋白质偶联的染色剂使细胞内的离子径迹可见来直接测试。然而,这两种方法都很耗时,而且只有在实验后才能验证撞击情况。这意味着实验中的靶向误差在实验过程中无法纠正。因此,我们开发了一种快速在线撞击验证方法,该方法在细胞照射前以±1微米的空间分辨率通过电子方式测量靶向精度。