Sahin Afsun, Kiratli Hayyam, Tezel Gaye Guler, Soylemezoglu Figen, Bilgic Sevgul
Department of Ophthalmology, Ocular Oncology Service, Hacettepe University School of Medicine, Ankara, Turkey.
Ophthalmic Res. 2007;39(1):40-4. doi: 10.1159/000097905. Epub 2006 Dec 11.
It was the aim of this study to assess the expression of vascular endothelial growth factor (VEGF)-A, matrix metalloproteinase (MMP)-9 and extravascular matrix patterns (EMPs) in iris and ciliary body melanomas and their correlations with histopathologic parameters.
The study was conducted on 3 iris and 15 ciliary body melanomas. All tumors were subjected to immunohistochemical techniques for VEGF-A and MMP-9 expressions, the presence of EMPs was assessed, and routine paraffin sections were stained with hematoxylin-eosin. Cell type, tumor localization, degree of pigmentation, necrosis, mitotic index, lymphocytic infiltration and sclera invasion were analyzed using light microscopy.
The mean patient age at the time of treatment was 43 years (range 19-69, median 39.5); 10 (55.6%) patients were males and 8 (44.4%) females. Histopathological cell types were spindle cells in 55.6%, mixed cells in 16.7%, and epithelioid cell types in 27.8% of tumors. Positive reaction for VEGF-A and MMP-9 was present in 66.7 and 72.3% of the tumors, respectively. Microvascular loops and/or networks were seen in 33.4% of the tumors, with the remaining 66.7% of tumors displaying one or more of the other patterns. Metastatic disease developed in only 1 patient during follow-up. Tumor cell type, tumor size, mitotic rate, degree of pigmentation and EMPs were not correlated with metastasis.
This study suggests that VEGF-A and MMP-9 were positive in the majority of iris and ciliary body melanomas. No correlation was found between VEGF-A and MMP-9 immunoreactivity and EMPs and occurrence of metastases in cases of anterior uveal melanoma.
本研究旨在评估血管内皮生长因子（VEGF）-A、基质金属蛋白酶（MMP）-9及血管外基质模式（EMP）在虹膜和睫状体黑色素瘤中的表达情况及其与组织病理学参数的相关性。
对3例虹膜黑色素瘤和15例睫状体黑色素瘤进行研究。所有肿瘤均采用免疫组织化学技术检测VEGF-A和MMP-9的表达，评估EMP的存在情况，并对常规石蜡切片进行苏木精-伊红染色。通过光学显微镜分析细胞类型、肿瘤定位、色素沉着程度、坏死情况、有丝分裂指数、淋巴细胞浸润及巩膜侵犯情况。
治疗时患者的平均年龄为43岁（范围19 - 69岁，中位数39.5岁）；10例（55.6%）患者为男性，8例（44.4%）为女性。组织病理学细胞类型中，55.6%为梭形细胞，16.7%为混合细胞，27.8%为上皮样细胞类型。分别有66.7%和72.3%的肿瘤VEGF-A和MMP-9呈阳性反应。33.4%的肿瘤可见微血管环和/或网络，其余66.7%的肿瘤表现为一种或多种其他模式。随访期间仅1例患者发生转移。肿瘤细胞类型、肿瘤大小、有丝分裂率、色素沉着程度及EMP与转移均无相关性。
本研究表明，大多数虹膜和睫状体黑色素瘤中VEGF-A和MMP-9呈阳性。在前葡萄膜黑色素瘤病例中，未发现VEGF-A和MMP-9免疫反应性与EMP及转移发生之间存在相关性。
