通过DNA光连接实现高度序列特异性的RNA末端标记
Highly sequence specific RNA terminal labeling by DNA photoligation.
作者信息
Yoshimura Yoshinaga, Noguchi Yuuki, Fujimoto Kenzo
机构信息
School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan.
出版信息
Org Biomol Chem. 2007 Jan 7;5(1):139-42. doi: 10.1039/b615715g. Epub 2006 Nov 28.
DOI:10.1039/b615715g
PMID:17164918
Abstract
We report the nonenzymatic terminal labeling of oligoribonucleotide (ORN) by using template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine ((CV)U) with high selectivity.
摘要
我们报道了通过5-羧基乙烯基-2'-脱氧尿苷（(CV)U）进行模板导向的光连接，以高选择性对寡核糖核苷酸（ORN）进行非酶末端标记。
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