Ogino Masayuki, Fujimoto Kenzo
The School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Nucleic Acids Symp Ser (Oxf). 2007(51):231-2. doi: 10.1093/nass/nrm116.
We now report template-directed photoligation using ODN containing alpha(C)U and beta(C)U at both terminal ends. By using this method, we performed template-directed photoligation in two positions at the middle of DNA and synthesized R-shaped DNA as novel structure. The isolated R-shaped DNA was characterized by MALDI-TOF MS and enzymatic digestion.
我们现在报道使用在两个末端都含有α(C)U和β(C)U的寡脱氧核苷酸（ODN）进行模板导向的光连接。通过使用这种方法，我们在DNA中间的两个位置进行了模板导向的光连接，并合成了具有新型结构的R形DNA。分离出的R形DNA通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和酶切进行了表征。
