通过α-5-氰基乙烯基脱氧尿苷实现可逆DNA光连接

Reversible DNA photoligation via alpha-5-cyanovinyldeoxyuridine.

作者信息

Ogino Masayuki, Yoshimura Yoshinaga, Nakazawa Akio, Saito Isao, Fujimoto Kenzo

机构信息

The School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):77-8. doi: 10.1093/nass/48.1.77.

DOI:10.1093/nass/48.1.77

PMID:17150486

Abstract

An efficient and reversible Template-directed Photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha-(CNV)U) was described. When ODN containing alpha-(CNV)U at the 3' end was photoirradiated with ODN containing thymidine at the 5' end in the presence of a template ODN, an efficient photoligation was observed. The ligated ODN can be split site-selectively to regenerate parent ODN by photoirradition at the 312 nm.

摘要

描述了一种使用α-5-氰基乙烯基脱氧尿苷（α-(CNV)U）对寡脱氧核苷酸（ODN）进行高效且可逆的模板导向光连接。当在模板ODN存在的情况下，对3'端含有α-(CNV)U的ODN与5'端含有胸苷的ODN进行光照射时，观察到了高效的光连接。通过在312 nm处进行光照射，连接的ODN可以进行位点选择性裂解以再生亲本ODN。