Matsuzawa H, Nirenberg M
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3472-6. doi: 10.1073/pnas.72.9.3472.
3':5'-cGMP levels of neuroblastoma N1E-115 cells increase as much as 200-fold upon activation of muscarinic acetylcholine receptors, resulting in intracellular cGMP concentrations greater than 600 pmol/mg of protein. The cells also have receptors for adenosine which mediate an increase in 3':5'-cAMP levels. Unexpectedly, prostaglandin E1 was found to increase the concentrations of both cGMP and cAMP. Carbamylcholine, adenosine, and PGE1 were added to cells separately and in pairs to determine the effect of one compound on cell responses to another. Reciprocal inhibition, unilateral inhibition, additive, and nonadditive responses were observed with respect to cGMP and cAMP levels when different pairs of receptors were activated simultaneously.
毒蕈碱型乙酰胆碱受体激活后,神经母细胞瘤N1E - 115细胞的3':5'-环鸟苷酸(cGMP)水平可增加多达200倍,导致细胞内cGMP浓度高于600 pmol/mg蛋白质。这些细胞还具有腺苷受体,可介导3':5'-环磷酸腺苷(cAMP)水平升高。出乎意料的是,发现前列腺素E1可增加cGMP和cAMP的浓度。分别及成对地向细胞中添加氨甲酰胆碱、腺苷和前列腺素E1,以确定一种化合物对细胞对另一种化合物反应的影响。当同时激活不同的受体对时,观察到cGMP和cAMP水平存在相互抑制、单向抑制、相加和非相加反应。